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Place the sampling grid in the
petri dish.
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Place your disc in the petri dish and cover with water from the sampling site (note: for very thick biofilms, you may need to reverse these two steps and place the grid on top of the disc).
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Use your random number table to select a grid to sample. To choose your starting point, close your eyes and touch the table with a finger.
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Going left to right, determine the next 3 digit number.
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Determine if it is between 1 and 100, inclusive. In other words, is it one of the numbered sites on our
sampling grid.
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If the number is within this range, locate this grid using the steromicroscope and record the number of different organisms attached to the disc (i.e., part of the biofilm).
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If the number is outside this range, go to step 3.
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Repeat steps 3-5 three times (i.e., take 3 random samples).
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If our number was 018, we would locate grid number 18.
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